Originally posted by papori
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In fact, what the paper says is:
"Combined with the existing strand-specific cDNA library preparation strategies, such as T7 RNA polymerase–based in vitro transcription and dUTP second-strand synthesis strategies, it will be possible to recover the strandedness information for single-cell transcriptomes in the near future"
As I understand, it is implying that you could use T7-IVT to amplify into cRNA then from that create a cDNA library, with uracil incorporated into the second strand. This can then go into library prep, have the second strand degraded and finally PCR amplifed. It might give you enough material so to sequence whilst maintaining strandedness. Although IVT is a very 3' biased amplification, so there are probably going to be issues with this approach.
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