Hi,
Im a little confused with the results i got maybe someone here could help me on this
basically i did PCR and i got a band on the gel which was approx 225-300bp but when the pcr product it was sanger sequenced i recieved a 401 nucleotide long result which part of the gene for drosha.
sequence adapters maybe?
how could this be, the gel shows a thick band with the middle of the band aprox 262 base pairs.
what could cause this difference in result, it wasnt myself who ran pcr and sequencing but it was i who prepared the mixtures
thank you
Im a little confused with the results i got maybe someone here could help me on this
basically i did PCR and i got a band on the gel which was approx 225-300bp but when the pcr product it was sanger sequenced i recieved a 401 nucleotide long result which part of the gene for drosha.
sequence adapters maybe?
how could this be, the gel shows a thick band with the middle of the band aprox 262 base pairs.
what could cause this difference in result, it wasnt myself who ran pcr and sequencing but it was i who prepared the mixtures
thank you
Comment