A seemingly very simple question, but not one that I can find the answer to from experts or the literature.
I am using NanoString to look at pathway signalling in aggressive B cell Non-Hodgkin Lymphoma.
Our preliminary data showed very variable expression of the included HKs (ACTB, TUBB, GAPDH) so in future iterations we are going to use TBF which has been validated in one or two papers.
Any other ideas? Does anyone have experience regarding this?
I thought we could look through old affymetrix data for consistent expression of certain genes but our biostats person does not seem keen on this approach.
Any ideas very welcome.
I am using NanoString to look at pathway signalling in aggressive B cell Non-Hodgkin Lymphoma.
Our preliminary data showed very variable expression of the included HKs (ACTB, TUBB, GAPDH) so in future iterations we are going to use TBF which has been validated in one or two papers.
Any other ideas? Does anyone have experience regarding this?
I thought we could look through old affymetrix data for consistent expression of certain genes but our biostats person does not seem keen on this approach.
Any ideas very welcome.
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