Hi all,
Just wondering if anyone knows anything about doing this?
I read an article by Beck et al where they developed a method called 3SEQ for RNA-Seq from FFPE samples (using the Illumina GA) but this method concentrates only on the extreme 3` end of samples and therefore obviously misses out on a lot of upstream information.
I have read that single molecule sequencing is most suited to profiling mRNA from degraded samples and I am wondering if the Helicos or Pacific Bio machines would be suited to such analyses? Or will their techniques be 3`-biased also?
Any feedback would be greatly appreciated
Best,
Gavin
Just wondering if anyone knows anything about doing this?
I read an article by Beck et al where they developed a method called 3SEQ for RNA-Seq from FFPE samples (using the Illumina GA) but this method concentrates only on the extreme 3` end of samples and therefore obviously misses out on a lot of upstream information.
I have read that single molecule sequencing is most suited to profiling mRNA from degraded samples and I am wondering if the Helicos or Pacific Bio machines would be suited to such analyses? Or will their techniques be 3`-biased also?
Any feedback would be greatly appreciated
Best,
Gavin
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