Any help would be appreciated?
Our group have performed recent whole genome/exome sequencing in tumor samples and now I would like to take a few of these variants (say maximum 50 amplicons) to:
1) Validate them in the original samples
2) Perform ultra-deep sequencing in an extension cohort of samples but using different amplicons for different samples (hope that makes sense!)
How would one do this...as majority of the targeted sequencing approaches focuses on a large panel of genes/exons in a large number of samples. We have experience of using Fluidigm but I don't need to multiplex that number of amplicons.
So for example, say I want to screen just 2 exons of KRAS in 10 samples but want a coverage of say 10,000x so I can study the clonal complexity and evolution. How would one do this?
Thank you!
Our group have performed recent whole genome/exome sequencing in tumor samples and now I would like to take a few of these variants (say maximum 50 amplicons) to:
1) Validate them in the original samples
2) Perform ultra-deep sequencing in an extension cohort of samples but using different amplicons for different samples (hope that makes sense!)
How would one do this...as majority of the targeted sequencing approaches focuses on a large panel of genes/exons in a large number of samples. We have experience of using Fluidigm but I don't need to multiplex that number of amplicons.
So for example, say I want to screen just 2 exons of KRAS in 10 samples but want a coverage of say 10,000x so I can study the clonal complexity and evolution. How would one do this?
Thank you!
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