Hi All,
I am new to the transcriptome sequencing field and I am learning to use Bowtie, Tophat and Cufflinks. I find that Cufflinks spits out RPKM and FPKM values which though very informative does not help my purpose since I am interested in the number of raw reads per gene. That is to say without normalization. Is there a way of figuring this out?
Thanks
Abhijit
I am new to the transcriptome sequencing field and I am learning to use Bowtie, Tophat and Cufflinks. I find that Cufflinks spits out RPKM and FPKM values which though very informative does not help my purpose since I am interested in the number of raw reads per gene. That is to say without normalization. Is there a way of figuring this out?
Thanks
Abhijit
Comment