Hi, I did bisulfite seq on the same library, first using MiSeq to check quality, and then using Hiseq to get more reads.
But the duplication level was 50% from MiSeq, and 95% from HiSeq, even though the exact same library was used.
Was this increase in duplicate caused by natural duplication, or too much sequencing coverage? Are there any other explanations? Thank you very much!
But the duplication level was 50% from MiSeq, and 95% from HiSeq, even though the exact same library was used.
Was this increase in duplicate caused by natural duplication, or too much sequencing coverage? Are there any other explanations? Thank you very much!
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