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  • RNAsequestion
    Junior Member
    • Jan 2015
    • 8

    Looking for collaboration for RNA seq data analysis

    Dear Bioinformaticians:

    The wisdom of the sages of NG Sequencers says that it is not advisable to put efforts to analyze the whole exome (EXON) sequence data to try to find gene fusion because of the chances being very slim. I am thinking of doing RNA seq of 3 tumors. Is somebody ready to analyze that for me for gene fusions. I am open to develop collaboration and the person will get due credit.

    Thanks in advance

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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