Dear Colleagues. I'm looking at small RNA fragments in my samples associated with or derived from mRNAs. I prefer to use the running window generator in SeqMonk because I can then annotate against various features without having to redefine the probes. The question is, what are the best settings to use? The libraries are typical NEBNext generated from 18-21 and 23-30 nt size fractionated RNA. I'm not normalising the read counts but I am rejecting duplicate reads. All advice greatly appreciated.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM