Using the ENCODE overlap rule, the top 40% of each peak file was compared to the entire set of 9042 peaks for the other factor. A 90%–91% overlap was observed, indicating that the majority of the highest-ranked peaks for each factor are contained within the peak set for the other factor. When the ENCODE overlap rule is applied to replicate data sets of the same factor, high-quality data sets often overlap by 80%–90%.
Thanks,
Lou