Hello!
We conducted library prep and sequencing with Illumina. During data analysis, bioinformatics claims that the variants for all 40 samples were exactly the same. The %PF was >80, % aligned was about 3.64 (read 1) and 3.54 (read 4), with a density of 235. Illumina tech support stated that the run quality is passing but what would cause the same variants to be called for every sample in the run?
We conducted library prep and sequencing with Illumina. During data analysis, bioinformatics claims that the variants for all 40 samples were exactly the same. The %PF was >80, % aligned was about 3.64 (read 1) and 3.54 (read 4), with a density of 235. Illumina tech support stated that the run quality is passing but what would cause the same variants to be called for every sample in the run?
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