Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    Sequencing small DNA fragments (25bp)

    Hi all,

    Just wondering if anyone has any experience of the following. I want to sequence a single fragment of RNA to confirm it is what I think it is. Any suggestions on how to go about it. The major problme is it is only 25 bases long. I can convert to DNA and have a 7bp primer but not sure where to go from here. I have access to illumina, pyromark Q24, ABI 3730. I have tried cloning the fragment with no success. Any other suggestions.
    Tags: None
Previous template Next

Latest Articles

Collapse
  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
The Adaptation of the Cell Cycle in Multiciliated Cells by seqadmin
Started by seqadmin, 06-07-2024, 06:58 AM
0 responses
13 views
0 likes
 Last Post seqadmin
by seqadmin
06-07-2024, 06:58 AM  
New Method for DNA Sequence Amplification by seqadmin
Started by seqadmin, 06-06-2024, 08:18 AM
0 responses
20 views
0 likes
 Last Post seqadmin
by seqadmin
06-06-2024, 08:18 AM  
New Tools Enhance Single-Molecule DNA Analysis with Minimal Samples by seqadmin
Started by seqadmin, 06-06-2024, 08:04 AM
0 responses
18 views
0 likes
 Last Post seqadmin
by seqadmin
06-06-2024, 08:04 AM  
SIX2 Protein Identified as a Key Player in Prostate Cancer Treatment Resistance by seqadmin
Started by seqadmin, 06-03-2024, 06:55 AM
0 responses
13 views
0 likes
 Last Post seqadmin
by seqadmin
06-03-2024, 06:55 AM  
View All
Working...
X