Hi All,
I'm fairly new to RNA.seq and newer to RNA.seq of FFPE tissues. And thought I'd tap into the knowledge base here.
We have a number of RNA samples derived from FFPE tissue, they have DV200 scores above 50% and 1ug or so total yield. We are looking to get as much information from them as possible and I was thinking of using the Illumina RNA Exome kit for library prep or another capture method to look at isoform changes.
Or should I just be looking at ribo-depletion and sequencing deep?
I'm fairly new to RNA.seq and newer to RNA.seq of FFPE tissues. And thought I'd tap into the knowledge base here.
We have a number of RNA samples derived from FFPE tissue, they have DV200 scores above 50% and 1ug or so total yield. We are looking to get as much information from them as possible and I was thinking of using the Illumina RNA Exome kit for library prep or another capture method to look at isoform changes.
Or should I just be looking at ribo-depletion and sequencing deep?
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