So I saw several papers referencing a method used by Sonah (2013) where they used a selective primer that has one or two additional bases to further reduce their GBS library. This step will further reduce complexity but increases the read depth.
My question is, how necessary/useful is this step for non-plant species? I know Sonah et al used that to improve depth coverage in soybean GBS libraries since plant genomes have lots of highly repetitive fractions. The thing is, I'm working with an insect genome and other studies on insects I've read also used selective primers though I haven't seen a justification for it other than citing Sonah's method.
My question is, how necessary/useful is this step for non-plant species? I know Sonah et al used that to improve depth coverage in soybean GBS libraries since plant genomes have lots of highly repetitive fractions. The thing is, I'm working with an insect genome and other studies on insects I've read also used selective primers though I haven't seen a justification for it other than citing Sonah's method.
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