Dear all,
Usually we find this kind of quality error of Solexa tag
Each four-numbers correspond to 1 base. Hence, the above
quality refer to length 3 tags (e.g. "tca").
My question are as follows:
Usually we find this kind of quality error of Solexa tag
Code:
-33 31 -40 -34 -40 -40 -40 40 27 -27 -40 -40
quality refer to length 3 tags (e.g. "tca").
My question are as follows:
- What is the reasonable way to find single number to represent each base? (e.g should we average the 4 figures or pick the highest score out of 4?)
- How can we interpret the figure? e.g. Is base with positive quality score is better than negative quality score?
- In general, how do people use this type of quality score information?
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