how to detect ori of plasmid

[biocc]

Moving to ....um...general?

You can guess at the origin of replication as the coverage may be higher there if you were to sequence a dividing population. I've seen this in bacterial datasets. I would guess that unless the plasmid is very large that you'd have a hard time detecting the overabundance of replication near the ori.

thanks. my plasmid in a bacteria is only 11k. the relation between coverage and ori is interesting. could you explain it. why it happened?

[ECO]

Moving to ....um...general?

You can guess at the origin of replication as the coverage may be higher there if you were to sequence a dividing population. I've seen this in bacterial datasets. I would guess that unless the plasmid is very large that you'd have a hard time detecting the overabundance of replication near the ori.

[biocc]

hi, I want to predict ori of a plasmid. but it sems there is not a good method for plasmid. we can use GC skew to find ori of genome. is it available for plasmid.
In addition, I found it is vary slowly to open up SEQanswers web in china. does anyone have the same question?
thanks