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  • DNATECH
    Member
    • Mar 2015
    • 31

    SPLIT-Seq Single-Cell Webinar and Grants

    The UC Davis DNA Technologies & Bioinformatics Cores and Parse Biosciences will be hosting a Split-Seq Webinar and offering a Single-Cell Grant.

    Learn more about the technology and the grant at a webinar on Tuesday, May 11th, at 11am PST.
    Please find the grant application & webinar registration here: https://www.parsebiosciences.com/ucd-grantprogram

    The Parse Biosciences Split-Seq technology works VERY differently from droplet-based technologies. It enables large-scale single-cell & single-nuclei gene expression studies.
    These Split-Seq features could be advantageous for some projects:
    - Split-seq works with fixed samples --> thus, no scheduling stress. Fixed samples are stable for months
    - No cell size limits
    - Tolerates some debris in the cell suspensions
    - Study up to 48 samples per run

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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