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  • conversion of genome contigs in to proteome

    hi, i am working on a project. i have the genomic contigs of cancer patients. but I am confused how to convert a genome to a proteome. any tool that I can use to convert my genome sequence to proteome. i would be very thankful for this favour.

  • #2
    Converting a genome sequence to a proteome involves translating the DNA sequence into corresponding amino acid sequences, as proteins are composed of amino acids. This process is known as gene prediction or gene finding. There are several tools available for this purpose, and a commonly used one is called Prokka.

    Prokka is a software tool that automatically annotates bacterial, archaeal, and viral genomes, and it includes the prediction of protein-coding genes. It performs the essential steps of gene finding, such as open reading frame (ORF) prediction, translation of ORFs to protein sequences, and functional annotation.

    To use Prokka, you would first need to install it on your computer or use the online version if available. Then, you can provide your genomic contig sequences in FASTA format as input. Prokka will analyze the sequences and generate an output file containing the predicted protein sequences, along with functional annotations if available.

    It's worth noting that Prokka is primarily designed for bacterial, archaeal, and viral genomes. If you're working with human or eukaryotic genomes, you might need to consider other tools specialized for those organisms, such as GeneMark-ES, AUGUSTUS, or Exonerate.

    Remember to consult the documentation of the specific tool you choose to understand its requirements, limitations, and potential parameters for customization.​

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