Dear all
I used wgsim to simulate illumina reads, and I got read1 and read2 fastq files.
But all bases of quality scores are "2"
222222222222222222222222222222222222222222222222222222222222222222222222222
@gi|49175990|ref|NC_000913.2|_26305_26624_0:2:0_2:0:0_b/1
GTTTTTGTGCCGGTGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTGCGGCGCACCGATATATG
+
222222222222222222222222222222222222222222222222222222222222222222222222222
@gi|49175990|ref|NC_000913.2|_967853_968177_1:7:3_2:3:0_c/1
TGACGATTACCGCATAAACCGACTTTAAGCACCCCGCTCGCTAACGCATACGCCCCGCCGGCAACCACCAGCCAT
What's wrong with my process
~/Simulation/samtools-wgsim$ wgsim -d 350 -s 30 -N 70000 -1 75 -2 75 /Simulation out.read1.fq out.read2.fq
or the situation is normal ?
I used wgsim to simulate illumina reads, and I got read1 and read2 fastq files.
But all bases of quality scores are "2"
222222222222222222222222222222222222222222222222222222222222222222222222222
@gi|49175990|ref|NC_000913.2|_26305_26624_0:2:0_2:0:0_b/1
GTTTTTGTGCCGGTGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTGCGGCGCACCGATATATG
+
222222222222222222222222222222222222222222222222222222222222222222222222222
@gi|49175990|ref|NC_000913.2|_967853_968177_1:7:3_2:3:0_c/1
TGACGATTACCGCATAAACCGACTTTAAGCACCCCGCTCGCTAACGCATACGCCCCGCCGGCAACCACCAGCCAT
What's wrong with my process
~/Simulation/samtools-wgsim$ wgsim -d 350 -s 30 -N 70000 -1 75 -2 75 /Simulation out.read1.fq out.read2.fq
or the situation is normal ?
Comment