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Hello
Just wanted to say "Hello" to everyone, and glad to see this wonderful site up and running as I will probably have many questions in the near future. I'm a post doctorate researcher, and have some experience with 454 sequencing of bacterial genomes. However, the genomes that I have experience were fairly small (~1.8 Mb) compared to the genomes that I'm now working with (~5.5 Mb), in addition the new genomes have lots of bacteriophage and inverted repeats. The genomes have been assembled [I]de novo[I] using paired end and whole shotgun sequencing, however there are still over 200 contigs per genome. I'm in the process of filling the gaps using PCR and Sanger sequencing, however any suggestions that people have about ways to assemble would be much appreciated.
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM -
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM