Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
This topic is closed.
X
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Hi all,
    I'm a PhD student working on predicting and modeling alternative splicing, identifying DEGs and differentially alternatively spliced genes.

    Comment


    • hi

      Hi, I am from southern California and do work on genomic and messenger RNA. Nice to see a forum where I can ask questions.

      Comment


      • Introduction

        Hello everyone I am a research fellow and new to this field. My current project needs knowledge about ChIP and RIP analysis. Apart from that can anyone give a perfect explanation on Cistrome and a protocol for carrying out these ChIP and RIP assays. Any suggestions would be a great help for me. Thank you

        Comment


        • NextGen for undergrads

          Hi All.
          I am an undergraduate educator and part of a new network that is trying to include NextGen sequencing into undergraduate research and the core curriculum. Organizing this network has been supported by a grant from NSF (NSF Award # DBI-1061893 ) . The network has recently become part of the Genome Consortium for Active Teaching (GCAT) and are known as GCAT-SEEK ( http://lycofs01.lycoming.edu/~newman/gcat-seek/ ).

          We are looking for sequencing organizations who might be interested in partnering with groups of undergraduate colleges throughout the U.S. to facilitate getting raw NextGen sequence to support undergraduate research and education. The goal is to include novel next-generation DNA sequence analysis in ongoing undergraduate research, so that these research activities can be incorporated into core courses within the undergraduate life sciences curriculum. While there is plenty of unanalyzed sequences available in multiple databases, there is also an abundance of evidence if the education literature (e.g. http://jmbe.asm.org/index.php/jmbe/a...view/133/pdf_9) that if the teacher and the students do not share a sense of ownership in the data and the research question then laboratory and classroom exercises based on that data move from being exciting opened-inquiry to busy work. Our goal is to excite the next generation of researchers (currently undergraduates) using NextGen sequencing and all of its ”cool” applications.

          If any sequence facility is interested in this type of outreach, please let me know.
          Mike Boyle

          Comment


          • Hello from an long-time NGS fan

            I've been working on software and the analysis of NGS sequencing data since before the first 454 sequencer came out, but I'm sorry to say I'd never registered at SEQanswers before!

            Thanks Eric for running such a great site for the community, and I'm happy to help contribute to the forums.

            Jon Sorenson

            (ex software lead AB SOLiD, ex bioinformatics lead PacBio RS, current genome analyst @ Locus Development)

            Comment


            • Hi,
              My name is Kacper. I'm work in Poland at National Research Institute of Animal Production and have just started working with the HiSeq from Illumina.
              Regards,
              Kacper

              Comment


              • Hi every body my name is charles, this is my very first time and i am learning how to map shot reads of RNASEQ from Illunima. i hope to get help from you good guys. cheers

                Comment


                • Hello everyone!

                  My name is Angela and I just started working as a Molecular Biologist at the National Institutes of Health. My lab is trying to use next generation sequencing technologies (Illumina GAIIx and maybe Ion Torrent in the future) for a genetic research study of eye genes. While I already have some experience with library preparation and sequencing, I'm realizing that I'm only scratching the surface.

                  I'm sure I'll have tons of questions and I hope to be able to help others with the knowledge I gain in the future.

                  ~Angela

                  Comment


                  • Hi there!!!

                    I'd just like to say to the forum. I'm really excited to start absorbing from the collective source of knowledge!!!

                    -Kieran

                    Comment


                    • Hi

                      Hi:

                      Looking for help with deep sequence analysis on the Macintosh and found some helpful pointers here already. Thanks and hopefully I will be able to contribute some help as well.

                      Cheers

                      Comment


                      • Hello

                        I am a postdoc balancing the pros and cons between Illumina and 454 RNA-seq methods. I am so glad SEQanswers exists to support questions, and will probably be asking some questions soon

                        Comment


                        • Hey community!

                          I'm a PhD student in Germany and am now starting to try NGS analysis as our University just installed its first Illiumina HiSeq2000 and had its first run recently. I'm not a Bioinformation by training but hope to get helpful insight in this community.
                          So far, I played with bowtie, Seqmonk and FastQC, which is imho a good introduction to NGS data analysis because they are rather easy to handle.

                          In particular, I'm working on histone modifications and the corresponding enzymes in neuronal tissue in health an disease. So I'll deal with ChIP-seq and RNA-seq at present and in the near future.

                          Thanks in advance for answering beginners questions!

                          Comment


                          • Hi all,

                            I am a graduate who has this year made the move out of the lab and into an office cubicle to work in bioinformatics. I have dabbled in bovine rumen metagenomics (using 454 and illumina) and now have a job assembling fungal endophyte genomes (using illumina HiSeq2000). I am really enjoying my foray into bioinformatics and this forum has been such a great resource. Thanks for creating such a supportive and vibrant online community!

                            Cheers,
                            Sophie

                            Comment


                            • Hello all,

                              I'm a recent graduate and a new research technician working at the University of Wisconsin-Madison. Our lab does lots of pyrosequencing using our GS Junior to genotype non-human primate MHC genes. Additionally we're moving into whole genome analysis, which will become a big part of my job, using Illumina data. My background is primarily in bacteriology, but have always had an interest in bioinformatics and have been gradually learning the ropes. This website is a great resource and I'm sure this will not be my last post.

                              Thanks,
                              Gabriel

                              Comment


                              • Hello everyone!....my name is Isabel, I am doing a PhD in Molecular Genetics in Barcelona, Spain. I am working with maize actually in ChIP-Seq of a TF and I am glad to be in this community

                                Comment

                                Latest Articles

                                Collapse

                                • seqadmin
                                  Choosing Between NGS and qPCR
                                  by seqadmin



                                  Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
                                  10-18-2024, 07:11 AM
                                • seqadmin
                                  Non-Coding RNA Research and Technologies
                                  by seqadmin




                                  Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.

                                  Nobel Prize for MicroRNA Discovery
                                  This week,...
                                  10-07-2024, 08:07 AM

                                ad_right_rmr

                                Collapse

                                News

                                Collapse

                                Topics Statistics Last Post
                                Started by seqadmin, Yesterday, 05:31 AM
                                0 responses
                                10 views
                                0 likes
                                Last Post seqadmin  
                                Started by seqadmin, 10-24-2024, 06:58 AM
                                0 responses
                                20 views
                                0 likes
                                Last Post seqadmin  
                                Started by seqadmin, 10-23-2024, 08:43 AM
                                0 responses
                                48 views
                                0 likes
                                Last Post seqadmin  
                                Started by seqadmin, 10-17-2024, 07:29 AM
                                0 responses
                                58 views
                                0 likes
                                Last Post seqadmin  
                                Working...
                                X