Hi,
I am new in sequencing and bioinformatics and trying to get the terms right.
I am doing WGS of e. coli genomes on Illumina HiSeq machines.
Read length: Is that the length of the DNA fragment between the tags being replicated on the flow cell?
Inserts: The actual sequence you get from the machine?
Fragment size: Not sure...
Cycles: I have samples run in 75 and 100 cycles, meaning what?
Please help me, so confused...
I am new in sequencing and bioinformatics and trying to get the terms right.
I am doing WGS of e. coli genomes on Illumina HiSeq machines.
Read length: Is that the length of the DNA fragment between the tags being replicated on the flow cell?
Inserts: The actual sequence you get from the machine?
Fragment size: Not sure...
Cycles: I have samples run in 75 and 100 cycles, meaning what?
Please help me, so confused...
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