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  • chip-seq and repetitive elements

    hello everyone,
    i am a new post-doc at Yale university, from Nigeria an received my PhD in Biomedical Science
    i have a technical question i was hoping i could get some assistance with.
    I am interested in starting a Chip-Seq study to determine transcription factor binding sites in differentiated Human Endometrial Stromal cells. I am interested in binding sites at repetitive elements and was wondering about the certainty of mapping the binding sites to specific regions of the human genome.
    Last edited by eze; 08-18-2009, 09:01 AM.

  • #2
    "repetitive elements"... what kind of repeats?

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    • #3
      preliminary studies have shown that the TF that i am interested in can bind to DNA transposable elements . the problem is; when mapping the reads to the human genome, what is the certainty that it will map to the right site. is it possible that i will get multiple hits?
      Last edited by eze; 08-19-2009, 08:13 AM.

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