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anyone interested to exchange data and/or utilities to analyze/organize
influenza-sequence databases ?
influenza-sequence databases ?
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suggest collaboration
Perhaps this sequence collection may be interesting and useful joined in collaborative effort such as the Pandemic Influenza Digital Archive as described here.
researchfestival.nih.gov/sessiondetail.php?IC_ID=2656 -
I had had no idea that such a thing existed.
Why was it kept secret ?
Aren't scientists being appropriately "rewarded" for publishing
their stuff ?
Alas, for this pandemic it comes too late.
Good however that it turned out to be "mild".
Maybe Moren's decision to publish was made at a time
when it looked worse...Comment
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see also
High Throughput Full Genome Sequencing of 2009 Swine H1N1 Isolates From World Wide Origins
Huo-Shu Houng, Ph.D., Viral Disease, Walter Reed Army Institute of Research
Novel 2009 inluenza A (H1N1) is a new flu virus of swine origin that was first detected in Mexico and the United States in March/April 2009. We systematically investigate the potential of ultra-deep pyrosequencing to determine and assemble full genome sequences of H1N1 viruses from world-wide geographic origins. A robust RT-PCR protocol was established to efficiently amplify across the boards of all 8 2009 novel H1N1 RNAs into sufficient cDNA quantities using the Roche 454 FLX system.
at http://www.healthtech.com/Conference....aspx?id=91502
and
Title: High-throughput Sequencing Pipeline for Complete Influenza A Viral Genomes from Clinical Samples: Nucleotide Polymporphisms, Antiviral Resistance, Ultra-Depth Sequencing Coverage and Clinical Relevance
Dr. Huo-Shu Houng, Division of Viral Diseases, Walter Reed Army Institute of Research, USA
at http://chinapharm.blog.163.com/blog/...0942382139816/Comment
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are the sequences available ?
I have what is at genbank, but it's tedious to format,align,correct,sort etc.
it would be useful to share such improved databases
and provide upatesComment
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as it happens the Walter Reed people had to resequence all their
influenza sequences. Apparently it's not so easy with high-throughput,
to assign the fragments to the correct virusesComment
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Thank you for the update. Do you have any online references to updated material? Can you provide us with any further description on the progress of database curation on this topic and how such databases might be accessed?Comment
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I assume they will submit updated sequences
but it can take a while
watch here for daily flu-updates:
ftp://ftp.ncbi.nih.gov/genomes/INFLUENZA/updates/Comment
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See also
See also Infuenza Research Database for detailed sequences information
Comment
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Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM -
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...10-07-2024, 08:07 AM
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