Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #31
    Hi Jim,

    IGV is a great tool! I use it all the time. I have a question about the “coverage”. I hope to view all the reads mapped to a region, according to IGV, the depth of the reads is about 1000. However, I am not sure whether IGV has displayed all the 1000 reads, since if I use the “expanded” view, the number of the gray bars (I assume each gray bar represents one read) doesn’t seem to be 1000. I tried the change the “Maximum read depth” under “Alignment” of the “Preferences”. However, the number of the gray bars doesn’t seem to increase. I probably missed something on the user’s manual. Thank you very much!

    Comment


    • #32
      Hi, IGV downsamples to "max read depth" for display, but counts all reads for the coverage., Changing the "max read depth" value it is the correct way to see more, you didn't miss anything. It should refresh automatically, did it seem to reload after changing the value?

      Comment


      • #33
        Hi Jim, thanks! I closed the brower and reload the file, and now I can see all the reads.

        Comment


        • #34
          OK, thanks for reporting back. That is a bug, it should have reloaded without having to quit. I'll note it and fix it in an upcoming release.

          Jim

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Recent Advances in Sequencing Analysis Tools
            by seqadmin


            The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
            Today, 07:48 AM
          • seqadmin
            Essential Discoveries and Tools in Epitranscriptomics
            by seqadmin




            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
            04-22-2024, 07:01 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Today, 07:17 AM
          0 responses
          11 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-02-2024, 08:06 AM
          0 responses
          19 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-30-2024, 12:17 PM
          0 responses
          20 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-29-2024, 10:49 AM
          0 responses
          28 views
          0 likes
          Last Post seqadmin  
          Working...
          X