Hello, my name is Ksenia. I am writing a master's thesis. To do this, I need to understand what methods of probe synthesis companies use, such as Agilent/IDT, etc. I know classical synthesis methods consisting of 4 stages, some types of printing, PCR synthesis and synthesis using ligase (chemical-enzymatic). But for example, Agilent receives probes based on SurePrint technological equipment operating on the principle of ink-jet printing. What does the synthesis scheme look like in this case? I have assumptions that some stages of classical organic synthesis are absent in this case, because they are unnecessary and in order to increase the speed and yield of synthesis.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM