Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
This topic is closed.
X
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Bacterial WGS on Ion Torrent

    Hi There,..
    We were running a bacterial WGS using Ion fragment library prep kit protocol.
    After fragmentation of sample, we performed adaptor ligation and
    did size selection of libraries using simple AGE gel exclusion of 200-400bp region from smears (due to unavailability of other size selection options)
    We quantified libraries using Ion Universal Library Quantification Kit.

    Picture of AGE gel is attached.
    Quantification results were up to the mark giving individual libraries quantities above 100pm.

    After equalization, we put our libraries on IonChef for Templating, and then on Ion S5 XL for sequencing.
    Results gave median length of 50bp and after assembly a contiguous sequence of 500bp per sample.

    Need troubleshooting on this case.

    Looking forward to hearing from NGS experts.
    Thanks in anticipations.

    Best Regards.
    Attached Files

  • #2
    was that the Ion Torrent™ Ion Xpress™ Plus Fragment Library Kit (Cat. No. 4471269) for preparing library or he Ion Plus Fragment Library Kit (Cat. No. 4471252) because the later involves physical fragmentation via sonication method .

    Comment


    • #3
      Surely the same as mentioned above. But we did enzymatic fragmentation by Ion Shear fragmentation kit because no physical fragmentation strategy was available.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        The Impact of AI in Genomic Medicine
        by seqadmin



        Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
        02-26-2024, 02:07 PM
      • seqadmin
        Multiomics Techniques Advancing Disease Research
        by seqadmin


        New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

        A major leap in the field has
        ...
        02-08-2024, 06:33 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 02-28-2024, 06:12 AM
      0 responses
      30 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-23-2024, 04:11 PM
      0 responses
      74 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-21-2024, 08:52 AM
      0 responses
      86 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-20-2024, 08:57 AM
      0 responses
      71 views
      0 likes
      Last Post seqadmin  
      Working...
      X