Hi to all, I'm new in this group. I discovered this place thanks to the Nick Loman and Mick Wantson's article http://www.nature.com/nbt/journal/v3.../nbt.2740.html. Friends, I need help with two issues:
- First: I need a software or script for grouping sequences (from a Fasta file) in haplotypes. If anyone know something about this, I will be greatifull with you.
- Second: The next year I will sequence some amplicons with a MiSeq sequencer, but I have never handle a sequence machine. Of course, as biologist I know the Sanger's method, but this machine have advanced tricks for mount the sequence. I need help please, if anyone could explain me how I must mount the sequences I will be greatifull too.
PD: My amplicons have between 400 and 3000 bp.
- First: I need a software or script for grouping sequences (from a Fasta file) in haplotypes. If anyone know something about this, I will be greatifull with you.
- Second: The next year I will sequence some amplicons with a MiSeq sequencer, but I have never handle a sequence machine. Of course, as biologist I know the Sanger's method, but this machine have advanced tricks for mount the sequence. I need help please, if anyone could explain me how I must mount the sequences I will be greatifull too.
PD: My amplicons have between 400 and 3000 bp.
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