Hello - genome gaps - SEQanswers

Al'Thor

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Join Date: Jul 2014

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Hello - genome gaps

07-03-2014, 06:02 AM

Hi everyone,

New to this site and relatively new to this field also.

I am using the MiSeq platform (nextera XT, v2 chemistry, 500 cycles) for resequencing of bacteria to a well defined reference genome.

My question - assuming the prep is good, 20-40x coverage and there are good Phred scores what is the likelyhood of assembly gaps remaining that can't be fixed informatically and thus would need additional sequencing?

Cheers
Al'Thor

Thanks
Al'Thor
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