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  • KroSeq
    Junior Member
    • Dec 2014
    • 4

    #1

    Single-cell analysis: from DNA to RNA world

    Hi there,

    I'm new to this community, so this is to say hello and - at the same time - thanks to those providing helpful hints and information...

    I was doing single/rare-cell DNA analysis and joined a team working on single-cell RNA issues...
    ... now that I got introduced to the RNA world (which caused some extra adaptation in the lab as my DNA-lab-reference-system is kind of resisting to hand over to RNA) I more or less feel comfortable and can dig into things.

    The reason for being here is that I'm working on single-cell RNA sequencing which is why I started to follow the Smart-Seq2 protocol published earlier this year. Happy about the info I already got by surfing through the posts I might come up with one or two myself...

    Looking forward to interact ...

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  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
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    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
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