Greetings
I am a newcomer to the field of sequencing with advanced technology. I do not own and do not plan to own 454 or Illumina equipment, but I want to use this technology (by contracting services) in order to get the genome sequence of a fungus.
My fungus (Penicillium purpurogenum) has a genome of about 21.2 Mbp (according to electrophoretic karyotype). I have been told that with 454 technology I can obtain a rough sequence of good enough quality to allow me to make gene searches (using peptide sequence data obtained by MS or from similar sequences from databases).
I have contacted some providers who perform the sequencing process. However, I have a question concerning the alignment of the raw data. Do I have to cotract this too? Is there public domain software that can be used for this purpose?
I look forward to suggestions and comments.
My fungus (Penicillium purpurogenum) has a genome of about 21.2 Mbp (according to electrophoretic karyotype). I have been told that with 454 technology I can obtain a rough sequence of good enough quality to allow me to make gene searches (using peptide sequence data obtained by MS or from similar sequences from databases).
I have contacted some providers who perform the sequencing process. However, I have a question concerning the alignment of the raw data. Do I have to cotract this too? Is there public domain software that can be used for this purpose?
I look forward to suggestions and comments.