Tweet
Hi,
I am Psotdoc planning to use NGS to do RNA-seq in next 4 monthes. I looked at some post here and feel this is a realy good forum.
Some people suggested me to use Illumina to do RNA-seq instead of SOLiD. However, I only have limited amount of RNA and Helicos looks a good option for the RNA input requirement. Is there any thorough comparation of pros and cons from different methods such as Helicos, Illumina, SOLid, PacBio. In addition, could anyone suggest me some good softwares for analyze the NGS data. I only know GenomeQuest and DNAstar. I liked GenomeQuest but haven't try DNAstar. Is there any more options?
Thanks!
Sunny
I am Psotdoc planning to use NGS to do RNA-seq in next 4 monthes. I looked at some post here and feel this is a realy good forum.
Some people suggested me to use Illumina to do RNA-seq instead of SOLiD. However, I only have limited amount of RNA and Helicos looks a good option for the RNA input requirement. Is there any thorough comparation of pros and cons from different methods such as Helicos, Illumina, SOLid, PacBio. In addition, could anyone suggest me some good softwares for analyze the NGS data. I only know GenomeQuest and DNAstar. I liked GenomeQuest but haven't try DNAstar. Is there any more options?
Thanks!
Sunny