Tweet
Hello. It would be great if you comment on this issue with ncRNA.
Currently, we are using Illumina HiSeq System to deep seq patients' samples. RNA-seq was finished successfully, and now we are seeking a way to perform deep seq for non-coding RNAs.
Witht HiSeq, it is possible to read up to 100bp, so we are thinking the following 2 possibilities:
(1) Cut the gel for <500bp and seq for middle and small ncRNAs.
(2) Cut the gel for <50bp and seq for microRNAs.
With (1), we think that there are contamination by tRNA and small ribosomal subuints, which leads to low resolution.
Do you know of any other ways?
Thanks for your help.
Shizuka
Currently, we are using Illumina HiSeq System to deep seq patients' samples. RNA-seq was finished successfully, and now we are seeking a way to perform deep seq for non-coding RNAs.
Witht HiSeq, it is possible to read up to 100bp, so we are thinking the following 2 possibilities:
(1) Cut the gel for <500bp and seq for middle and small ncRNAs.
(2) Cut the gel for <50bp and seq for microRNAs.
With (1), we think that there are contamination by tRNA and small ribosomal subuints, which leads to low resolution.
Do you know of any other ways?
Thanks for your help.
Shizuka