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RNA sequencing.
Methods Mol Biol. 2011;759:125-32
Authors: Waern K, Nagalakshmi U, Snyder M
Abstract
This chapter describes the RNA sequencing (RNA-Seq) protocol, whereby RNA from yeast cells is prepared for sequencing on an Illumina Genome Analyzer. The protocol can easily be altered to use RNA from a different organism. This chapter covers RNA extraction, cDNA synthesis, cDNA fragmentation, and Illumina cDNA library generation and contains some brief remarks on bioinformatic analysis.
PMID: 21863485 [PubMed - in process]
More...
RNA sequencing.
Methods Mol Biol. 2011;759:125-32
Authors: Waern K, Nagalakshmi U, Snyder M
Abstract
This chapter describes the RNA sequencing (RNA-Seq) protocol, whereby RNA from yeast cells is prepared for sequencing on an Illumina Genome Analyzer. The protocol can easily be altered to use RNA from a different organism. This chapter covers RNA extraction, cDNA synthesis, cDNA fragmentation, and Illumina cDNA library generation and contains some brief remarks on bioinformatic analysis.
PMID: 21863485 [PubMed - in process]
More...