Syndicated from PubMed RSS Feeds
Ultra-deep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in SIV-infected macaques.
J Virol. 2009 Jun 10;
Authors: Bimber BN, Burwitz BJ, O'Connor S, Detmer A, Gostick E, Lank SM, Price DA, Hughes A, O'Connor D
Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultra-deep pyrosequencing to create a comprehensive picture of CD8+ T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a complex pattern of viral variants previously undetected. This increased sensitivity enabled detection of acute CD8-TL escape as early as 17 days post-infection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.
PMID: 19515775 [PubMed - as supplied by publisher]
More...
Ultra-deep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in SIV-infected macaques.
J Virol. 2009 Jun 10;
Authors: Bimber BN, Burwitz BJ, O'Connor S, Detmer A, Gostick E, Lank SM, Price DA, Hughes A, O'Connor D
Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultra-deep pyrosequencing to create a comprehensive picture of CD8+ T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a complex pattern of viral variants previously undetected. This increased sensitivity enabled detection of acute CD8-TL escape as early as 17 days post-infection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.
PMID: 19515775 [PubMed - as supplied by publisher]
More...