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Systematic characterization of protein-DNA interactions.
Cell Mol Life Sci. 2011 Jan 5;
Authors: Xie Z, Hu S, Qian J, Blackshaw S, Zhu H
Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.
PMID: 21207099 [PubMed - as supplied by publisher]
More...
Systematic characterization of protein-DNA interactions.
Cell Mol Life Sci. 2011 Jan 5;
Authors: Xie Z, Hu S, Qian J, Blackshaw S, Zhu H
Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.
PMID: 21207099 [PubMed - as supplied by publisher]
More...