Thanks for posting your dual indexing primer sequences. I was planning on using the Nextera barcode sequences but wanted to double check. The Illumina rep said that the posted sequences are the sequences as read by the MiSeq. Does that mean that the 5'-3' barcode sequences on the primer should all be reverse complemented from what Nextera provides?
Also, do you have any feelings on standard desalted primers vs HPLC or PAGE purified primers? I've found that people have strong feelings either way but would like to get away with the cheapest method that works.
Thanks
Originally posted by koadman
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