Hey guys,
Quick question. I am running a TruSeq small RNA library for a collaborator on our MiSeq. The libraries have a polyA tail at the 3' end. I was wondering how much phiX I should spike in in order to get more diversity throughout the polyA stretch. Does anyone have any experience with that?
Thanks!
Quick question. I am running a TruSeq small RNA library for a collaborator on our MiSeq. The libraries have a polyA tail at the 3' end. I was wondering how much phiX I should spike in in order to get more diversity throughout the polyA stretch. Does anyone have any experience with that?
Thanks!