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  • #1

    which colors correspond to which bases

    Hi

    I am trying to run an Illumina MiSeq reaction in a tube. Does anybody know which dyes correspond to each particular (A, T, G , C) reversible terminator.

    Did anybody try to run the reaction in a tube/PCR machine. Any advice on a reference where the conditions (cycle duration, temperature) are mentioned would be highly appreciated.

    Thanks in advance!!
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