It only tells you this in some of the NEB manuals (e.g. NEB #E7335S) but I would imagine the adaptor is the same concentration in all their kits. Certainly this works very consistently for me.
When you use less adaptor, it is also possible to use less USER enzyme and do smaller reactions for end repair, A-tailing, ligation etc.
The whole library synthesis scales down very well, and works for me with tiny amounts of input DNA. However I am sequencing viral genomes which are relatively small... so we don't need much DNA to cover the genome many times.
Originally posted by kbushley
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