Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • jorondo
    Junior Member
    • Mar 2022
    • 6

    #1

    Several studies with all bases having super high quality (100% question mark ASCII)

    I ran into several studies where the samples all seem to have "perfect" quality score of 63 (? over all bases). First time, I thought the samples quality had been tampered with, but then I found 6 more studies sequenced on various illumina platforms that had the same feature. Some examples: SRP410115, SRP403740, SRP423365, SRP424298, SRP425931, SRP434700​.

    @SRR22424534.1 1/1
    GTACTGATACACAAAGTAATGACCTGCAATCCACGATGATTGCTAGGCTTATATTGTTTCTTTTTCATTTTTAAAAATCTTTACTAACTTAAAACCAAAAGGGCACAATGCCCCAAATTGTCTGCAAAGATACGAATAAACGAATAGATA
    +
    ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
    @SRR22424534.2 2/1
    AGCGTATTGGAAAGGATTATTGTCCTCGCTATAATTCTTACTAGAATACACCTTGTCTGAGTGAATAAGGCTCAACTGATTGCCATCAGCACGTAAGAAATAAGCATACGGAATAATGAACGGAGTGTGATCAGGCTCAATACATTGGG
    +
    ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
    ​

    I've heard of quality binning but what I've found doesn't explain a score that high, nor does it explain why ALL bases are perfect; in some cases I still find a decent amount of human DNAs in the samples so I don't expect them to have been QC'd. I can't find anything on the illumina website. Anyone has an explanation?

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    Today, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
68 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...