Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • dannyhi321
    replied
    Originally posted by cmbetts View Post
    The MiSeq at my former company had issues where there would be a poor seal with the flowcell gaskets, resulting in an accumulation of salts where the flowcell seats on the instrument. Occasionally wiping it down with some dI water when salts became visible seemed sufficient to prevent issues.

    More recently, when we had a MiSeq installed at my current company, the FAS specifically mentioned cleaning any salt buildup there. I think she recommended using ~70% ethanol, but my memory is fuzzy and we haven't had to deal with it on this instrument that I'm aware of.
    Thanks for that - why didn't they just fix the leak?

    Leave a comment:


  • cmbetts
    replied
    Originally posted by dannyhi321 View Post
    I have been having problems with leakage from one of my Miseq flow cells. There is pitting on the heater/slide holder, from salt/fluid build up it seems and resulting erosion - I get contradictory responses from Illumina, some tech people say it is a know problem, others say not!

    I have another system and that does not show the same.

    Anyone else experienced this problem?

    Thanks
    The MiSeq at my former company had issues where there would be a poor seal with the flowcell gaskets, resulting in an accumulation of salts where the flowcell seats on the instrument. Occasionally wiping it down with some dI water when salts became visible seemed sufficient to prevent issues.

    More recently, when we had a MiSeq installed at my current company, the FAS specifically mentioned cleaning any salt buildup there. I think she recommended using ~70% ethanol, but my memory is fuzzy and we haven't had to deal with it on this instrument that I'm aware of.

    Leave a comment:


  • dannyhi321
    started a topic Miseq Flow cell leakage Problems

    Miseq Flow cell leakage Problems

    I have been having problems with leakage from one of my Miseq flow cells. There is pitting on the heater/slide holder, from salt/fluid build up it seems and resulting erosion - I get contradictory responses from Illumina, some tech people say it is a know problem, others say not!

    I have another system and that does not show the same.

    Anyone else experienced this problem?

    Thanks

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-25-2024, 11:49 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-24-2024, 08:47 AM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
62 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Working...
X