A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and sequencing), and CLIP-Seq (cross-linking immunoprecipitation and sequencing) experiments. To use the NEXTflex Small RNA-Seq Kit V3, the RNA of interest must have a 5' monophosphate and 3' hydroxyl. Using the following steps posted on our website, these modifications can easily be added to most RNA molecules with T4 polynucleotide kinase (PNK).
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM