Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • New Applications Forums

    In an effort to increase the organization of the discussions and make things easier to find...I've created a new set of "Applications" forums:

    Library Generation

    Epigenetics

    RNA Sequencing

    De novo discovery

    My intent is to segment the purely scientific/biology discussions away from the previously instrument provider-focused areas. I think this organization offers more opportunity for people with different instruments to cross-pollinate, as regardless of what chemistry/instrument produces the data, the biology remains (roughly!) the same.

    I welcome comments, suggestions, mocking, laughing, whatever!

  • #2
    Just added Genomic Resequencing

    Comment


    • #3
      Well done ECO, I can see this being of particular benefit to people new to NextGen sequencing that probably don’t own a machine but are interested in applying for grants etc; it will definitely improve accessibility to the site. I was wondering what you or anyone else thought about including a “metagenomics” section and (the application of most interest to me) a “molecular diagnostics” section.

      Further to this – in the “about me” section we can define ourselves by “location” and “instrument”, would it be useful for members to be able to define themselves also by “specialism/field/interest” e.g. bioinformatics or epigenetics? Just a thought.

      Comment


      • #4
        Thanks jOn...good thoughts. I will definitely work towards the new forums you mentioned.

        I've been toying with a few ideas on how to improve the user profiles. Most specifically reducing the emphasis on "what instrument do you have". I don't think I want to restrict the field of interests to a list or menu (because I don't know all of the possibilities, and if I did it would be an immense list), so I'd have to open it up to being a user filled area.

        Good thoughts, keep 'em coming.

        Comment


        • #5
          Eco

          Is there a separate forum for sequence Annotation? anybody have suggestions for (microbial) genome annotation tools to use after assembling contigs/genomes?

          Thanks

          RudyS

          Comment


          • #6
            Originally posted by RudyS View Post
            Eco

            Is there a separate forum for sequence Annotation? anybody have suggestions for (microbial) genome annotation tools to use after assembling contigs/genomes?

            Thanks

            RudyS
            I am looking for one too.. Rudy, please pass along in case you stumble on something
            --
            bioinfosm

            Comment


            • #7
              Originally posted by RudyS View Post
              Eco

              Is there a separate forum for sequence Annotation? anybody have suggestions for (microbial) genome annotation tools to use after assembling contigs/genomes?

              Thanks

              RudyS
              Originally posted by bioinfosm View Post
              I am looking for one too.. Rudy, please pass along in case you stumble on something
              Hey guys,

              I'm happy to create such a forum if there is enough interest...is this not something that could belong in the bioinformatics forum? Maybe it's time to think about subdividing that forum? I'm not eager to do that as it forces casual browsers to visit many smaller forums...but it's worth discussing....

              Comment


              • #8
                ECO

                i dont see much "bio" in the bioinformatics forum ... mostly still questions about how to assemble DNA ... i did see a post from bioinfosm from almost a year ago!
                1/17/08
                "Just wanted to initiate some bioinfo discussions ... makes up a major chunk of this high throughput sequencing effort .. after getting the sequence
                Any members here having experience with the data .. using it with some open access or in-house tools for mutation analysis, etc."

                sooo it looks like two separate groups of problems ... maybe the people doing the annotation are biologists? i have found B2G useful

                but wonder how other people deal with large annotation problems ... actually my problem is relatively small ... 5 MBase microbial genome

                RudyS

                Comment


                • #9
                  ECO

                  i dont see much "bio" in the bioinformatics forum ... mostly still questions about how to assemble DNA ... i did see a post from bioinfosm from almost a year ago!
                  1/17/08
                  "Just wanted to initiate some bioinfo discussions ... makes up a major chunk of this high throughput sequencing effort .. after getting the sequence
                  Any members here having experience with the data .. using it with some open access or in-house tools for mutation analysis, etc."

                  sooo it looks like two separate groups of problems ... maybe the people doing the annotation are biologists? i have found B2G useful

                  but wonder how other people deal with large annotation problems ... actually my problem is relatively small ... 5 MBase microbial genome

                  RudyS

                  Comment


                  • #10
                    Originally posted by bioinfosm View Post
                    I am looking for one too.. Rudy, please pass along in case you stumble on something
                    I agree, would be great to have a talk about sequence annotation!

                    I will make a thread here as ECO suggested http://seqanswers.com/forums/showthr...=3854#post3854

                    Stegger

                    Comment

                    Latest Articles

                    Collapse

                    • seqadmin
                      Current Approaches to Protein Sequencing
                      by seqadmin


                      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
                      04-04-2024, 04:25 PM
                    • seqadmin
                      Strategies for Sequencing Challenging Samples
                      by seqadmin


                      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                      03-22-2024, 06:39 AM

                    ad_right_rmr

                    Collapse

                    News

                    Collapse

                    Topics Statistics Last Post
                    Started by seqadmin, 04-11-2024, 12:08 PM
                    0 responses
                    17 views
                    0 likes
                    Last Post seqadmin  
                    Started by seqadmin, 04-10-2024, 10:19 PM
                    0 responses
                    21 views
                    0 likes
                    Last Post seqadmin  
                    Started by seqadmin, 04-10-2024, 09:21 AM
                    0 responses
                    16 views
                    0 likes
                    Last Post seqadmin  
                    Started by seqadmin, 04-04-2024, 09:00 AM
                    0 responses
                    46 views
                    0 likes
                    Last Post seqadmin  
                    Working...
                    X