66 results in 0.0025 seconds.
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Whole genome assembly of cDNA with Illumina paired-end sequencing
Does anybody know an opensource software package for whole genome assembly of cDNA with Illumina paired-end sequencing? I'm really looking for an assembler... -
Position less than 0 from eland sorted
I was trying to use findpeaks on eland results using the 'separateelandsortedreads.jar' and it pointed out -ve coordinate mapped reads
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Anyone with Solexa RNA-seq 150+bp for non-model sps with no close reference genome?
Hi,
I am working on a method to cluster and assemble RNA-seq data for species that don't have a close genome reference. For example, a... -
Clusters-reads correlation?
Hi all,
I am involved in a sequencing project using Illumina/Solexa and I'm analyzing the data. I'm a little confused because the person... -
compare distribution of coverage in solexa data
Hi (i'm new in this forum and new in sequencing analysis, please be patient
)
I’m comparing some solexa re-sequence experiment in which... -
paired-end question
Hi,
I am trying to figure out Solexa paired end read output. Can someone please tell me if my toy example is correct?
1)... -
De novo assembly of human genomes with massively parallel short read sequencing
http://genome.cshlp.org/content/earl...1.109.abstract
Next-generation massively parallel DNA sequencing technologies... -
The sequence and de novo assembly of the giant panda genome
Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled... -
% unmapped reads
Hi all,
For any solexa run, we see 60-80% passing filter reads, of which 70-90% map to the reference sequence. There are quite a few reads... -
Assembling Solexa data using Newbler 2.3 Beta
Has anyone attempted to de-novo assemble 100 bp Solexa reads with Newbler 2.3 beta yet?
I would be curious to know what other people think... -
AMOScmp-shortReads-alignmentTrimmed
We have been trying to complete a hybrid de-novo assembly of Solexa / 454 / sanger BAC's using AMOScmp-shortReads-alignmentTrimmed;
After... -
Snp discovery without a reference
I have paired-end (76bp) output from a GA in which I would like to try snp discovery. The hiccup is there is no reference genome for my specie.
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ERANGE: map to transcriptome
Hi All,
I am attempting to get ERANGE up and running but have a few questions before I commit more time to it.
Specifically, after... -
RPKM and multiple reads, tophat and cufflinks
My understanding of the RPKM calculation in Tophat is that it includes multi-reads that match < 40 times in the genome (by default).
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Bioinformatics Computer: List your specs
Hi All,
In reading the forums, it seems like many people are having questions that involve computer power... How much RAM, how many processors,...
