Hello, I have some questions about the server data accessible through IGV
1. When you upload files from the server "Body Map 2.0 (Illumina...
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Data from servers Accessible on IGV
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WGCNA eigengene correlation
I'm using the WGCNA package in R to analyse RNA sequencing data. I've hit a point follwing the tutorial, and adapting it to my data, where I don't understand...
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How to get expression of one gene from 10 RNA-Seq datasets?
Hello guys,
I am trying to fetch gene expression level of one gene from 10 different samples in human and mouse. I think its very straight...
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How to Filter Significant Genes??
Hi Everyone,
I am using cummeRbund to do some DE analysis and have 3 samples each having 5 biological replicates. Right now, the number...
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Gene expression analysis-comparsion
I have normal tissue (control A, control B) and abnormal tissue (treatment A, treatment B). I need to do differential gene expression analysis. How can...
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Normalize Expression Matrix
I have expression matrix where rows represent samples and columns represent time points. and values represent absolute counts of expression. I notice...
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DEXSeq - Counting with HT-seq at the exon level
Hi!
I'm currently looking for differently expressed isoforms and got curious about the behaviour of HT-seq when counting exons. Basically,...
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Correlate expression levels with phenotypic measure?
Dear all.
I've been spending lots of time searching for an answer to this question, going through papers and discussing with people.
...
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Differential isoform expression in cuffdiff: how does it work?
I am running an analysis in CuffDiff using a GFF file of annotations from FlyBase (www.flybase.org). The GFF file contains annotation data for genes,...
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Gene expression of duplicate genes
I have some polyA(minus) RNAseq data and would like to quantify the gene expression of Pol3 transcribed genes. Well, quite a lot of these are duplicate...
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Cuffdiff problem output!!!
i have some problem related to cuffidiff output, i used this command for replicates:
cuffdiff -o diff_out -b <genome.fa> -p 10 -L CT,SS...Last edited by ALOK N PATEL; 11-27-2013, 03:49 AM.
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Can I use a small subset of transcripts as a reference for RNA-seq analysis
Instead of using all the transcripts as reference for the RNA-seq analysis, can I use a small subset which I am interested? I compared the results, of...
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Enrichment analysis with DAVID tool - question
Hi All,
I am still new in the field and I have a question please.
I have a dataset of gene expressions and I am running...Last edited by ComputGuy; 09-23-2013, 11:23 AM.
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Postdoctoral research fellow at Harvard analyzing next-generation sequencing data
Description
A post-doctoral position in human/statistical genomics is available in the laboratory of Dr. J. Danny Muehlschlegel, Department of...