Hi all,
there's a lot of chat about extraction tekkers to get HMW DNA so i think we've got that covered; however what i can't find is...
156 results in 0.0033 seconds.
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What's the best strategy for sampling to get high molecular weight DNA
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Sequel Sequencing Costs - Microbial Genomes
Howdy,
Tried to post this once, and it disappeared.
I have been tasked with assembling a per genome cost of Sequel sequencing... -
bluepippin size selection for SMRTbells
Hello,
at which step of the 10kb protocol for preparation of SMRTbell libraries do you run BluePippin 20kb cassette for size selection?... -
PacBio reagents
If a box of PacBio polymerase binding reagents is left at room temperature and allowed to warm to ambient temperature is it now useless? -
Problems of assembly polishing with RS_Resequencing.1 protocol
Hi all,
I’m experiencing a difficulty in getting a complete bacterial genome from PacBio sequencing data using the SMRT Analysis protocols.... -
Metagenomic Assembly from PacBio reads
Hi everyone,
I am trying to assemble a bacterial genome using PacBio reads and short illumina reads from a metagenomic pool of both eurkaryotic...Last edited by lmbrutscher; 04-03-2017, 12:21 PM. -
variant calling from pacBio Sequel data
I am struggling with variant calling in 'Re-sequencing' mode using SMRT v4 and so far found that the VCF format generate by SMRTv4 is not valid.
...Last edited by splaisan; 03-25-2017, 06:29 AM. -
Removing duplicate PacBio reads
Hi all,
I think the thread title says it all but below are a bit more details about my problem:
I have used PacBio sequence...Last edited by AgatheJ; 02-13-2017, 05:00 AM. -
Uneven coverage across scaffold
Hi,
I have run the SMRT-Portal RS_HGAP_Assembly.2 protocol.
I am getting a single whole chromosome assembled (~4.4Mb) but there is... -
AlignQC - Long-read alignment quality reporting software
Hello all, I haven't started many threads so please redirect me to the right place if I'm not supposed to self-promote here, or there is a tools introduction... -
PacBio ENA SRA submission without bas.h5 files?
Dear all,
I've been sent some PacBio data from a collaborator that we now wish to submit to ENA. I've read that the way to do this is to... -
Hybrid assembly with low coverage PacBio libraries
Hi everyone,
We are working on sequencing the genomes of a few bacteria and microalgae. We recently had a PacBio run which provided pretty... -
Advice on getting good PacBio libraries
Hi everyone,
We are having issues getting good quality PacBio libraries from Bacteria and microalgae. Specifically, we got low yield (76... -
trouble shooting PB assembly generating a larger than expected contig
So I've got a newly assembled genome that was sequenced using PacBio sequencing and obtained >100x coverage. It was assembled with HGAP.3 and for the... -
Demultiplexing PacBio sequences
Hi all,
I have the following problem:
I have a dataset containing circurlarized and linear DNA. It was amplified with two pairs of primers...
