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bluepippin size selection for SMRTbells

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  • bluepippin size selection for SMRTbells

    Hello,

    at which step of the 10kb protocol for preparation of SMRTbell libraries do you run BluePippin 20kb cassette for size selection? I am preparing gDNAs for complete sequencing.

  • #2
    Current 10kb protocol does not include a Pippin size selection step but if you want to include it then it will be after ligation clean ups. You might follow a Sequel 10 and 20kb hybrid protocol.

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    • #3
      With PacBio 10kb protocol, we got the library sizing around 7kb on agarose gel, no need to do size selection.

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