Hello!
I am using 454 Roche Junior, I have few questions about reads of sff file.
I have splitted a SFF file into individual SFF file (according...
18 results in 0.0023 seconds.
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SFF file - primers
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MINT Adapter for RNAseq
Dear Friends,
While reading the newbler manual, I found that there are MINT adapter in 454 RNA seq data. If any one knows the... -
Geneious for transcriptome alignment
Hi there,
I am new to NGS data and bioinformatics. But SEQanswers seems like a good place to start. Here is my problem for you :-)
...Last edited by toreoe; 01-31-2013, 05:39 AM. -
454 contings blastn no hits found!!!
Hi everyone,
I'm fresh in the forum and a newbie in bioinformatics.
I'm trying to blast some contigs to use the output in MEGAN. I installed... -
454 re-scoring
Hi there!
I am a little confused about the re-scoring option on sfffile. In the manual, v2.6 of the Data Analysis package (2011), says:...Last edited by CPCantalapiedra; 03-20-2012, 09:59 AM. -
Some problem about 454 gs flx+
hello!
I have a problem about GS FLX+(the latest instrument).
1. we recovery the capture beads which have enriched DNA a few days ago,... -
Copy number analysis using 454 data
Hi,
I am pretty new to sequence analysis in general, but I was wondering if anyone know how to identify amplifications and deletions from... -
How to do phylogenetic analysis with 25000 sequences?
Hi,
I'm trying to employ 454 in phylogenetic research. We have 10 different sequences (markers) amplified among 19 species (ca. 1/16 plate) for... -
MIRA assembly with MID barcodes for 454?
MIRA assembly with MID barcodes for 454?
How can you do it?? -
SRA files
Hi there!
Does anyone know how to create the SRA file to submit in the NCBI database?? My read data input are four .sff files, which I... -
How many functionally-assigned reads is enough?
Hi,
We are analyzing some 454 results annotated by MG-RAST v2. We have 6 samples in each category, e.g. A1, A2,...,A6 for category A and...Last edited by idoerg; 04-25-2011, 10:47 AM. -
how to call snps with samtools from 454.bam
Hi there,
I really like samtools for variant calling on Illumina.bam. However, now I have a GS Junior 454 data set. I aligned the reads... -
best strategy for rRNA from shotgun?
Hi,
I have a 454 metagenomic sample, from which I would like to extract species frequency & diversity, among other things. I am currently...Last edited by idoerg; 01-18-2011, 07:29 AM. -
How to limit the “Reference Mapper” Variants to CDS regions?
Hello!
Does someone knows if is there a way to limit the “Reference Mapper” Variants to coding regions still using RefGen & SNP131... -
solution for incomplete key sequence 454 flx jr amplicon?
Does anyone have a solution for the following problem: We routinely perform 454 amplicon sequencing. In a recent 454 flx jr amplicon sequencing run, the...
