Can I use the accepted_hits.bam of "tophat2 --fusion-search" to calculate the gene expression level?
Usually I just use tophat...
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tophat-fusion result
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accepted_hits.bam attributes
where can I find an explanation about all the attributes in the accepted_hits.bam file I got after running tophat --fusion-search?
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How can I determine the mapping rates of tophat output such as accepted_hits.bam?
I used TopHat to run the same RNA-Seq data with different -r/--mate-inner-dist and --mate-std-dev.
Here are the parameters:
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Tophat2 specify output file
Hi All,
This is a basic question wrt Tophat2 output. I want to name the output of tophat in the ./tophat/ to another name apart from "accepted_hits.bam"....